Orateur
Description
Study of fibroblast contractility on 2D and 3D soft gels
Sara Faour1,2, Cyrille Vezy1, Stephanie Salesse2, Stephane Dedieu2 and Rodolphe Jaffiol1
1 L2n, EMR CNRS 7004, UTT, Troyes, France
2 MEDyC, UMR CNRS 7369, URCA, Reims, France
Email: sara.faour@utt.fr
Fibroblast activation is a multi-step process defined by increased contractile properties and associated processes (increased ECM production, tissue remodelling, proliferation...). In the presence of persistent stimuli from cancer lesions, these fibroblasts become CAFs (Cancer-Associated Fibroblasts) that are pro-tumorigenic cells that can chemically and mechanically remodel the tumor micro-environment, promoting the proliferation and invasion of cancer cells [1]. A growth factor (TGF- β - a key mediator in activation) was used to activate two different subsets – normal fibroblasts (WPMY-1) and activated fibroblasts (exp-CAF1 [2]).
This activation was verified by the implementation of a functional assay using 3D gel composed of type-1 collagen. As the involvement of matrix stiffness has become more apparent in the differentiation of fibroblasts, hydrogels of different stiffness (1 kPa – 100 kPa) were prepared to mimic physiological and pathological conditions [3]. The stiffness of these hydrogels, embedded with micrometric beads, was characterized through active microrheology using optical tweezers to define the frequency-dependent viscoelastic modulus G*(ω).
[1] R. Kalluri, Nat. Rev. Cancer, 16 (2016), 582–598.
[2] Y. Kojima et al. PNAS, 107, (2010) 20009–20014.
[3] M. Carrancá et al. Journal of Biomedical Materials Research. Part A, 109 ,(2021) 926–937.
| Affiliation de l'auteur principal | 1. L2n, EMR CNRS 7004, UTT, Troyes, France 2. MEDyC, UMR CNRS 7369, URCA, Reims, France |
|---|
